labeling thick sections

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#7784

From: GREG VAUGHN MARTIN
Date: Thu, 18 May 1995 00:00

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Hey Folks -- We are trying to immuno-label 20um frozen sections of fixed
liver through the entire section thickness.  We have tried long (up to
overnight) incubation with primary antibody, included saponin or triton
(to 0.2%) and still have problems getting the antibodies into the middle
of the sections.  Does anyone have any protocols that they have used
successfully for similar specimens?  The tissue was embedded in
PVP-Sucrose prior to sectioning, and the sections can be either mounted
on slides or free-floating.  Please respond directly to me at
gmartin@welchlink.welch.jhu.edu.   Thanks in advance,
                                                Greg Martin
                                                Cell Biology and Anatomy
                                                Johns Hopkins School of Med.

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From: GREG VAUGHN MARTIN <gmartin@WELCHLINK.WELCH.JHU.EDU>
Subject: labeling thick sections
Date: 1995/05/18
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