pH sensitive fluorescent dyes

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1 total messages Started by Ferbs Fri, 28 Oct 2005 11:15

#63

Author: Ferbs
Date: Fri, 28 Oct 2005 11:15

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Dear all,

I am looking to find a pH sensitive fluorescent dye that exhibits a
greater fluorescence in low pH situations (as the concentration of H
ions increases I want it to be brighter).  This is the opposite of the
response to pH that most dyes show (e.g. fluorescein).  Probes have a
dye called lysosensor yellow/blue I think that does what I'm looking
for, however it is excited in the UV region and I really want one that
can be excited on most confocals (e.g. 488 nm or 568 nm).  Does anyone
have any suggestion of a suitable dye and where I get some?

Ta

Andy B.

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