need ideas for methods to kill off/ablate cells in chick embryo

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2 total messages Started by Michael Levin Fri, 23 Sep 1994 16:11

#4447

Author: Michael Levin
Date: Fri, 23 Sep 1994 16:11

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   I am looking for ideas/references on good ways to do the following:
I need to kill off (ablate) groups of cells in the early
(gastrulation) chick embryo. The embryo is in culture, so access to
the cells is not a problem. What are some ways (besides surgery) to
get rid of a particular group of cells? One thing I have done already
is to label the cells with DiI. Can some sort of photo-treatment be
used afterwards to kill off only cells that got it? What else have
people used? Please email mlevin@husc7.harvard.edu with any ideas.
Thanks.

Mike Levin

Re: need ideas for methods to kill off/ablate cells in chick embryo

#4448

Author: Rene Jansen
Date: Sun, 25 Sep 1994 21:26

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Michael Levin (mlevin@husc7.harvard.edu) wrote:

:    I am looking for ideas/references on good ways to do the following:
: I need to kill off (ablate) groups of cells in the early
: (gastrulation) chick embryo. The embryo is in culture, so access to
: the cells is not a problem. What are some ways (besides surgery) to
: get rid of a particular group of cells? One thing I have done already
: is to label the cells with DiI. Can some sort of photo-treatment be
: used afterwards to kill off only cells that got it? What else have
: people used? Please email mlevin@husc7.harvard.edu with any ideas.
: Thanks.

One method that has been used is label with lucifer yellow and then
blast the cell with a laser or some other high-intensity lightsource.
I can get you references tomorrow (I'm not in the lab now). Email me
if interested. 

Rene Jansen

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