Re: Tryptophan from PAGE gel hydrosylate

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Article #2263

#2263

From: jose
Date: Mon, 11 Jun 2001 21:05

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ckf wrote:

> I read your method for trypsin digests of PAGE samples. Is the method (and
> subsequent reverse phase HPLC) compatible with SDS from denaturing gels or
> is the method only compatible with native PAGE

i think i use it with SDS containing PAGE gels, too. it may be worth
your while to investigate the use of a heartier protease, one that is
SDS resistant, if you suspect thats a problem.

jose nazario				jose@cwru.edu

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From: jose <jose@biochemistry.cwru.edu>
Newsgroups: sci.chem,sci.chem.analytical,bionet.molbio.proteins
Subject: Re: Tryptophan from PAGE gel hydrosylate
Date: Mon, 11 Jun 2001 17:05:51 -0400
Organization: Case Western Reserve University, Cleveland, OH, USA
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