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Article #2264Re: Tryptophan from PAGE gel hydrosylate
From: Mitchell Isaacs
Date: Tue, 12 Jun 2001 02:00
Date: Tue, 12 Jun 2001 02:00
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"ckf" <craig@fulton52.freeserve.co.uk> wrote in message news:9g2vaf$i26$1@news5.svr.pol.co.uk... > jose > > I read your method for trypsin digests of PAGE samples. Is the method (and > subsequent reverse phase HPLC) compatible with SDS from denaturing gels or > is the method only compatible with native PAGE If you check out the technical bulletin for promega trypsin, (http://www.promega.com/tbs/tb512/TB512.pdf) you will see that it is resistant to mild denaturing conditions, eg 0.1% SDS. Cheers, Mitchell
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X-Google-Language: ENGLISH,ASCII-7-bit X-Google-Thread: fd9c4,26e8510285c04612 X-Google-Attributes: gidfd9c4,public X-Google-Thread: f4886,26e8510285c04612 X-Google-Attributes: gidf4886,public X-Google-Thread: f59b0,26e8510285c04612 X-Google-Attributes: gidf59b0,public X-Google-ArrivalTime: 2001-06-11 19:00:35 PST Path: arc1-ca.pugleaf.net!archiver1.google.com!newsfeed.google.com!newsfeed.stanford.edu!news-spur1.maxwell.syr.edu!news.maxwell.syr.edu!news.stealth.net!intgwpad.nntp.telstra.net!news-server.bigpond.net.au!not-for-mail From: "Mitchell Isaacs" <misaacs@spam_me_not.student.usyd.edu.au> Newsgroups: sci.chem,sci.chem.analytical,bionet.molbio.proteins References: <108aed20.0106050833.66a24275@posting.google.com> <3B1E787C.5AEE1870@biochemistry.cwru.edu> <9g2vaf$i26$1@news5.svr.pol.co.uk> Subject: Re: Tryptophan from PAGE gel hydrosylate Lines: 15 X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.50.4522.1200 X-MimeOLE: Produced By Microsoft MimeOLE V5.50.4522.1200 Message-ID: <6JeV6.125347$ff.935285@news-server.bigpond.net.au> Date: Tue, 12 Jun 2001 02:00:34 GMT NNTP-Posting-Host: 144.132.191.58 X-Complaints-To: news@bigpond.net.au X-Trace: news-server.bigpond.net.au 992311234 144.132.191.58 (Tue, 12 Jun 2001 12:00:34 EST) NNTP-Posting-Date: Tue, 12 Jun 2001 12:00:34 EST Organization: BigPond Internet Services (http://www.bigpond.net.au)