🚀 go-pugleaf

I'm trying to do a zymographic experiment, where I copolymerize a protein
substrate into my polyacrylamide gel, then run a cell extract to look at the
MW of a protease that digests the substrate (it leaves a hole).
	Anyway, my problem is that my substrate is only about 40 kD, so it
migrates a significant distance during the electrophoresis.  I've heard that
you can conjugate the substrate to linear polyacrylamide so that it will stay
in place (the conjugated complex has a MW ca. 10^6).  Until I can get my hands
on some pre-made linear polyacrylamide, can anyone suggest a way to prepare
my own? 

P.S.	I don't have tons of substrate, which is why I'm co-polymerizing the
substrate in the gel instead of diffusing it into the gel post-electrophoresis.
-- 
______________________________________________________________________________
Lou Hom >K'93		"It has been suggested that...gene splicing and cloning 
lhom@ocf.berkeley.edu    are so simple that one can successfully carry them out 
		         in [any] modern kitchen. In spite of my considerable
 Quote by 		 respect for the potential of food processors and radar
     B.R. Levin  	 ranges, I very much doubt this to be the case."