homoserine lactone hydrolysis

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Article #2324

#2324

From: Ben Davis
Date: Tue, 04 Apr 1995 00:00

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Hi,

We need to hydrolyse the homoserine lactone formed after CNBr cleavage of a
protein; has anyone got a protocol for doing this ? I suspect its an
alkaline hydrolysis I want, but I haven't got many ideas as to the exact
conditions ...

thanks a lot

Ben 



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______________________________________________________________________________

Ben Davis,
MRC Protein Function and Design,
Cambridge, UK
______________________________________________________________________________

"They can make me do it, but they can't make me do it with dignity."

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From: bjd12@cus.cam.ac.uk (Ben Davis)
Subject: homoserine lactone hydrolysis
Date: 1995/04/04
Message-ID: <3lrjr8$5qd@lyra.csx.cam.ac.uk>
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organization: University of Cambridge, England
keywords: homoserine, hydrolysis
newsgroups: sci.chem,bionet.molbio.proteins,bionet.molbio.methds-reagnts