🚀 go-pugleaf

rem@nite.toppoint.de (Rolf Elak) writes:

>In <2odajf$b82@nuscc.nus.sg> scip3156@leonis.nus.sg (Nobody) writes:
>>Hi,

>>Suppose I have the primary structure of a protein, is it possible to 
>>calculate a realistic tertiary structure of the protein?  How is the 
>>tertiary structures of proteins determined when drawn in a book?

>As others pointed out, there is no easy way to determine the t.s. of 
>a protein. As for the structures shown by x-ray or nmr-determination its
>IMHO questionable if they show the "right" structure, i.e. the structure 
>they would show in a cell environment. The t. s. of a protein is depending
>not only on the protein itself, but also on its environment. It is IMHO
>a *possible* structure that is shown by x-ray and nmr, but not necessarily
>the biologically achieved structure. Can someone provided a little in 
>depth information ? 

   The idea that x-ray or nmr structures of proteins are not the "right"
structures is probably not correct. Globular proteins have been shown to 
have essentially the same structures in a variety of quite different 
environments.  Many enzymes have biological activity under these conditions
etc etc.

!-----------------------------------------------------------------------
Simon M. Brocklehurst
Cambridge Centre for Molecular Recognition
Department of Biochemistry
University of Cambridge
Cambridge
UK

E-mail: s.m.brocklehurst@bioc.cam.ac.uk