Stable mRNA's?

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6 total messages Started by J�rgen Borreb�k Mon, 07 Jun 1999 00:00

Author: J�rgen Borreb�k
Date: Mon, 07 Jun 1999 00:00

10 lines
997 bytes

Hi!

I'm doing expression studies and need to check the stability of my
reference mRNA's when cells are subjected to treatment. Does anyone know
any mRNA's that's known to be stably transcribed under different
influences to the cells?
(like G3PDH, beta-actin or other usual kontrols)

Jorgen

Re: Stable mRNA's?

Author: Jarmo Schrader
Date: Mon, 07 Jun 1999 00:00

20 lines
1607 bytes

What cells? E.coli? Arabidopsis? Human? Drosophila?? Which tissue?

In article <jorgen.borrebak-0706991429450001@pkmac45.uio.no>,
jorgen.borrebak@basalmed.uio.no (J�rgen Borreb�k) wrote:

> Hi!
> 
> I'm doing expression studies and need to check the stability of my
> reference mRNA's when cells are subjected to treatment. Does anyone know
> any mRNA's that's known to be stably transcribed under different
> influences to the cells?
> (like G3PDH, beta-actin or other usual kontrols)
> 
> Jorgen

-- 
Jarmo Schrader     Umea, Sweden

Before replying to my e-mail delete 'removethis.' from the address!

Re: Stable mRNA's?

Author: Eike Staub
Date: Mon, 07 Jun 1999 00:00

15 lines
1336 bytes

Maybe you could take mRNAs of ribosomal proteins. They are used on
Gene Expression Experiments with some DNA Arrays.


"J�rgen Borreb�k" wrote:

> Hi!
>
> I'm doing expression studies and need to check the stability of my
> reference mRNA's when cells are subjected to treatment. Does anyone know
> any mRNA's that's known to be stably transcribed under different
> influences to the cells?
> (like G3PDH, beta-actin or other usual kontrols)
>
> Jorgen

Re: Stable mRNA's?

#10

Author: Alexander Brands
Date: Mon, 07 Jun 1999 00:00

25 lines
1960 bytes

> Hi!
>
> I'm doing expression studies and need to check the stability of my
> reference mRNA's when cells are subjected to treatment. Does anyone know
> any mRNA's that's known to be stably transcribed under different
> influences to the cells?
> (like G3PDH, beta-actin or other usual kontrols)
>
> Jorgen

Hi Jorgen,

I am afraid I do not know where the "crossed o" is hiding on my key board...

You could try Elongationfactor 1a.
In situ assessment of mRna accessibility in .....Gruber and Levine.
Histochem Cell Biol 1997. The article is about a in situ hybridization
problem, but should content interesting information for your application as
well.

Another method woul be Triplex-PCR...a lot of work to establish, but elegant
and powerful when it works (it does in our lab..). Check for GAPDH and
b-Actin simultaneously.

Alexander

Re: Stable mRNA's?

Author: J�rgen Borreb�k
Date: Tue, 08 Jun 1999 00:00

23 lines
1564 bytes

Sorry for not mentioning!
Caco2-cells (colon carcinoma) treated with certain lipids.
Jorgen


In article <Jarmo.Schrader-0706991856230001@d177.genfys.slu.se>,
Jarmo.Schrader@removethis.genfys.slu.se (Jarmo Schrader) wrote:

> What cells? E.coli? Arabidopsis? Human? Drosophila?? Which tissue?
> 
> 
> In article <jorgen.borrebak-0706991429450001@pkmac45.uio.no>,
> jorgen.borrebak@basalmed.uio.no (J�rgen Borreb�k) wrote:
> 
> > Hi!
> > 
> > I'm doing expression studies and need to check the stability of my
> > reference mRNA's when cells are subjected to treatment. Does anyone know
> > any mRNA's that's known to be stably transcribed under different
> > influences to the cells?
> > (like G3PDH, beta-actin or other usual kontrols)
> > 
> > Jorgen

Re: Stable mRNA's?

Author: Alexander Brands
Date: Wed, 09 Jun 1999 00:00

30 lines
2173 bytes

> Sorry for not mentioning!
> Caco2-cells (colon carcinoma) treated with certain lipids.

Good to know...We work with Caco2 as well and found in western blots that Villin
is a useful internal standard. Should work for mRNA, too.

Alexander


>
> Jorgen
>
> In article <Jarmo.Schrader-0706991856230001@d177.genfys.slu.se>,
> Jarmo.Schrader@removethis.genfys.slu.se (Jarmo Schrader) wrote:
>
> > What cells? E.coli? Arabidopsis? Human? Drosophila?? Which tissue?
> >
> >
> > In article <jorgen.borrebak-0706991429450001@pkmac45.uio.no>,
> > jorgen.borrebak@basalmed.uio.no (J�rgen Borreb�k) wrote:
> >
> > > Hi!
> > >
> > > I'm doing expression studies and need to check the stability of my
> > > reference mRNA's when cells are subjected to treatment. Does anyone know
> > > any mRNA's that's known to be stably transcribed under different
> > > influences to the cells?
> > > (like G3PDH, beta-actin or other usual kontrols)
> > >
> > > Jorgen

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