🚀 go-pugleaf

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Postdoctoral position as posted on Postdoc Jobs website:

http://www.PostdocJobs.com
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A position is immediately available for postdoctoral scientist to
study [Fe-S] proteins and novel oxidative stress response systems in
Azotobacter vinelandii.

Our interests on the [Fe-S] proteins are; 
1) to understand how polypeptide controls the reduction potential of
[Fe-S] clusters in [Fe-S] proteins. [Fe-S] cluster by itself has an
intrinsic potential based upon its chemical properties. When that
[Fe-S] cluster is sitting within a protein, the surrounding protein
environment will influence the reduction potential of the [Fe-S]
cluster. What factors of the protein structure influence the reduction
potential of the [4Fe-4S] cluster in ferredoxin I (FdI)?
2)to understand the mechanism of ability of [Fe-S] cluster to convert
from one form to another. Cluster interconversion is very important in
regulating the expression of genes in vivo in several enzymes. Using
molecular biology tools, we are trying to determine the protein factor
to control [Fe-S] cluster interconversion in FdI.

Aerobic organisms obtain energy by the oxidation of organic compounds
using oxygen as the final electron acceptor. As a by-product of this
process, reactive oxygen species generally are generated, with
potentially damaging consequences for the cell. The E1 subunit of
pyruvate dehydrogenase complex (PDHE1) in Azotobacter vinelandii seems
to respond to the intracellular increase in superoxide by inducing
sets of genes whose products either avert or repair the eventual
oxidative damage. In the system, 7Fe FdI appears to inhibit the
transcriptional activation through fpr promoter by binding to
DNA-bound PDHE1. However, little is known about how this novel system
responds an intracellular increase in superoxide. For this study, we
are employing proteomic techniques as well as molecular biology
techniques.

Ideal candidate should have strong background on these techniques of
Molecular Biology; cloning,site-directed mutagenesis, recombinant
protein expression, gel-mobility shift assay and DNA and RNA
analysis(Northern and Southern).

Please send curriculum vitae and the names and addresses of three
references by Jan. 15th.

For more information, please visit Postdoc Jobs website:

http://www.postdocjobs.com/jobs/302809.shtml

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