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Article #28Re: help: mounting medium for live cell fluorescent microscopy?
From: Rene
Date: Thu, 23 Sep 2004 09:02
Date: Thu, 23 Sep 2004 09:02
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I doubt it. Unless you really need live cells, you're best off fixing the cells and using glycerol based media. There are chemicals in use to prevent things(protists) moving, like methyl cellulose (no idea about auto-fluorescence) and narcotics, or you could try and find non-fluoreshing mesh material that would keep the cells in their place. You might look at embedding in Low melting point agarose or alginate (again, no idea about auto fluorescence). But for better optical properties you really need to change from water to glycerol or PVA based media. HTH, Rene. egf_repeats@hotmail.com (Patrick) wrote in message news:<d8a7e954.0409221027.7c67aa8c@posting.google.com>... > Hi, can anybody recommend a mounting medium for live cell fluorescent > microscopy except water? I tried glycerol based mounting medium > VECTASHIELD H-1000 but it bursted my cells immediately. Water is okay > but is there anything better than water in terms of preventing the > movement of the cells under microscope and better optical qualities? > Thanks.
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X-Google-Thread: 106237,fd0c149113634ef8 X-Google-Thread: 105a11,fd0c149113634ef8 X-Google-Thread: 114d69,fd0c149113634ef8 X-Google-Attributes: gid106237,gid105a11,gid114d69,public X-Google-Language: ENGLISH,ASCII-7-bit Path: arc1-ca.pugleaf.net!g2news1.google.com!postnews1.google.com!not-for-mail From: renevanwezel@hotmail.com (Rene) Newsgroups: sci.techniques.microscopy,bionet.molbio.methds-reagnts,bit.listserv.confocal Subject: Re: help: mounting medium for live cell fluorescent microscopy? Date: 23 Sep 2004 02:02:00 -0700 Organization: http://groups.google.com Lines: 20 Message-ID: <fc41d9d1.0409230102.54d8de6c@posting.google.com> References: <d8a7e954.0409221027.7c67aa8c@posting.google.com> NNTP-Posting-Host: 149.155.96.2 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Trace: posting.google.com 1095930120 27027 127.0.0.1 (23 Sep 2004 09:02:00 GMT) X-Complaints-To: groups-abuse@google.com NNTP-Posting-Date: Thu, 23 Sep 2004 09:02:00 +0000 (UTC)