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Article #27help: mounting medium for live cell fluorescent microscopy?
From: Patrick
Date: Wed, 22 Sep 2004 18:27
Date: Wed, 22 Sep 2004 18:27
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1301 bytes
Hi, can anybody recommend a mounting medium for live cell fluorescent microscopy except water? I tried glycerol based mounting medium VECTASHIELD H-1000 but it bursted my cells immediately. Water is okay but is there anything better than water in terms of preventing the movement of the cells under microscope and better optical qualities? Thanks.
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X-Google-Thread: 106237,fd0c149113634ef8,start X-Google-Thread: 105a11,fd0c149113634ef8,start X-Google-Thread: 114d69,fd0c149113634ef8,start X-Google-Attributes: gid106237,gid105a11,gid114d69,public X-Google-Language: ENGLISH,ASCII-7-bit Path: arc1-ca.pugleaf.net!g2news1.google.com!postnews1.google.com!not-for-mail From: egf_repeats@hotmail.com (Patrick) Newsgroups: sci.techniques.microscopy,bionet.molbio.methds-reagnts,bit.listserv.confocal Subject: help: mounting medium for live cell fluorescent microscopy? Date: 22 Sep 2004 11:27:14 -0700 Organization: http://groups.google.com Lines: 6 Message-ID: <d8a7e954.0409221027.7c67aa8c@posting.google.com> NNTP-Posting-Host: 128.42.86.13 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Trace: posting.google.com 1095877635 4386 127.0.0.1 (22 Sep 2004 18:27:15 GMT) X-Complaints-To: groups-abuse@google.com NNTP-Posting-Date: Wed, 22 Sep 2004 18:27:15 +0000 (UTC)