Vital stains for bacteria

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From: Brian Robertson
Date: Wed, 08 Mar 1995 09:34

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I am looking for a fluorescent vital stain to assess bacterial viability
in the FACS.

Fluorescene Diacetate (FDA), which fluoresces when the fluorescein is
released by the action of nonspecfic bacterial esterases in live cells, is
one possiblity, but is the wrong colour.

Does anyone know if, for example, rhodamine or texas red diacetate, or
some other colour is available? Or if there is another dye system around
which gives a colour that could be measured in something other than the
FITC channel?

Many thanks!

Brian Robertson

-- 
Dr. Brian D. Robertson
Dept. Medical Microbiology
St Mary's Hospital Medical School
Norfolk Place
London W2 1PG
U.K.

b.robertson@sm.ic.ac.uk

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From: b.robertson@ic.ac.uk (Brian Robertson)
Newsgroups: sci.techniques.microscopy,bionet.microbiology
Subject: Vital stains for bacteria
Date: Wed, 08 Mar 1995 09:34:34 +0000
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