Re: Vital stains for bacteria

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#25

From: Margaret Romine
Date: Thu, 09 Mar 1995 17:03

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In article <b.robertson-0803950934340001@cb-11.sm.ic.ac.uk>,
b.robertson@ic.ac.uk (Brian Robertson) wrote:

> I am looking for a fluorescent vital stain to assess bacterial viability
> in the FACS.
> 
> Fluorescene Diacetate (FDA), which fluoresces when the fluorescein is
> released by the action of nonspecfic bacterial esterases in live cells, is
> one possiblity, but is the wrong colour.
> 
> Does anyone know if, for example, rhodamine or texas red diacetate, or
> some other colour is available? Or if there is another dye system around
> which gives a colour that could be measured in something other than the
> FITC channel?
> 
> Many thanks!
> 
> Brian Robertson
> 
> -- 
> Dr. Brian D. Robertson
> Dept. Medical Microbiology
> St Mary's Hospital Medical School
> Norfolk Place
> London W2 1PG
> U.K.
> 
> b.robertson@sm.ic.ac.uk

Try molecular Probes, Inc. at 503-465-8300.  They have a large variety of
fluorescent probes.

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From: mf_romine@pnl.gov (Margaret Romine)
Newsgroups: sci.techniques.microscopy,bionet.microbiology
Subject: Re: Vital stains for bacteria
Date: Thu, 09 Mar 1995 09:03:48 -0800
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