🚀 go-pugleaf

Meir Ben-Levy <gimsyst@mail.netvision.net.il> wrote in article
<NEWTNews.876440189.23525.gimsyst@dialup.netvision.net.il>...
> 
> Dear Reader,
> We are looking for partners to develop a new Confocal technology.
> 
> Our group develops a new technology for Confocal Imaging by the
projection of 
> sinusoidal pattern with incoherent illumination, rather than the focused
laser 
> beam in the LSM technology. 
> 
> The advantages are ( as tested by a prototype):
> 1. Free choice of wave length using Halogen or arc lamp
> 2. Better Z resolution, we more than double the z resolution of the LSM.
> 3. Better x, y resolution, 10-15 % improvement.
> 
> I would like to hear your comments on this subject. 
> Dr. Meir Ben-Levy, GIM Systems

The principles, without getting into mathematical models: 

 The object is illuminated by a sine pattern, rather than the point
illumination of 
 the Confocal microscope. 
You can use any type of light source such as arc lamp or halogen.

 The technology is still in research, but we already have first results.

The focal (z) resolution (FWHM- full width of half maximum):
 with incoherent illumination of arc lamp, it is equal to 0.2um. 
 The resolution was measured and it also matches the theoretical depth of
focus of this type of illumination. 
 
We have excellent slices of semi-conductors lines in 0.25um technology. 
The height of such lines are approximately 0.25um.  

 The lateral (x, y) resolution:
 The sine illumination shifts down the spatial frequencies of the object,
so the optical microscope observe lower spatial frequencies. That way, it
is possible to "sneak in" high frequencies that normally cannot be observed
with optical microscopes. 

The results are limited by noise, in our tests we get 10-15% improvement
over the incoherent resolution of the microscope. 

This is equivalent to having a numerical aperture  of NA=1 in air or NA=1.5
in oil objectives.




Call Dr. Meir Ben-Levy for more details

Dr. Meir Ben-levy 
gimsyst@netvition.net.il

Meir Ben-Levy <gimsyst@mail.netvision.net.il> wrote in article
<NEWTNews.876440189.23525.gimsyst@dialup.netvision.net.il>...
> 
> Dear Reader,
> We are looking for partners to develop a new Confocal technology.
> 
> Our group develops a new technology for Confocal Imaging by the
projection of 
> sinusoidal pattern with incoherent illumination, rather than the focused
laser 
> beam in the LSM technology. 
> 
> The advantages are ( as tested by a prototype):
> 1. Free choice of wave length using Halogen or arc lamp
> 2. Better Z resolution, we more than double the z resolution of the LSM.
> 3. Better x, y resolution, 10-15 % improvement.
> 
> I would like to hear your comments on this subject. 
> Dr. Meir Ben-Levy, GIM Systems
> 
> 
> 


* Peter Verveer wrote 
------------------
Sounds exciting! Can you elaborate on the principles? Did you publish
something on it?


The principles, without getting into mathematical models: 

 The object is illuminated by a sine pattern, rather than the point
illumination of 
 the Confocal microscope. 
You can use any type of light source such as arc lamp or halogen.

 The technology is still in research, but we already have first results.

The focal (z) resolution (FWHM- full width of half maximum):
 with incoherent illumination of arc lamp, it is equal to 0.2um. 
 The resolution was measured and it also matches the theoretical depth of
focus of this type of illumination. 
 
We have excellent slices of semi-conductors lines in 0.25um technology. 
The height of such lines are approximately 0.25um.  

 The lateral (x, y) resolution:
 The sine illumination shifts down the spatial frequencies of the object,
so the optical microscope observe lower spatial frequencies. That way, it
is possible to "sneak in" high frequencies that normally cannot be observed
with optical microscopes. 

The results are limited by noise, in our tests we get 10-15% improvement
over the incoherent resolution of the microscope. 

This is equivalent to having a numerical aperture  of NA=1 in air or NA=1.5
in oil objectives.




Call Dr. Meir Ben-Levy for more details

Dr. Meir Ben-levy 
gimsyst@netvition.net.il

Eyal Peleg wrote:
> 
> Meir Ben-Levy <gimsyst@mail.netvision.net.il> wrote in article
> <NEWTNews.876440189.23525.gimsyst@dialup.netvision.net.il>...
> >
> > Dear Reader,
> > We are looking for partners to develop a new Confocal technology.
> >
> > Our group develops a new technology for Confocal Imaging by the
> projection of
> > sinusoidal pattern with incoherent illumination, rather than the focused
> laser
> > beam in the LSM technology.
>

<Snip>

>  The lateral (x, y) resolution:
>  The sine illumination shifts down the spatial frequencies of the object,
> so the optical microscope observe lower spatial frequencies. That way, it
> is possible to "sneak in" high frequencies that normally cannot be observed
> with optical microscopes.
> 

<Snip>

*ANY* resolution improvement in scanning microscopy can be explained
by the "mixing down" of higher spatial frequencies by the illumination
function, incoherent or not. So, not a really new idea. If you use a
ring pupil in the illumination optics, you can get very near to your
concept (only the highest spatial frequencies in the beam), while still
keeping (rotational) symmetry.

> This is equivalent to having a numerical aperture  of NA=1 in air or NA=1.5
> in oil objectives.
> 
Depends on what kind of optics you use to actually generate the
illumination function. If you are limited to NA 0.95 / NA 1.4 there, you
will also not really get to equivalent 1.0/1.5 in the end (besides the
difference being marginal). 


Joachim