Thread View: uchi.bio
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4 total messages
Started by Michael J. Fath
Mon, 02 Oct 1995 00:00
Mol Biologists, do any of you use precast gels?
Author: Michael J. Fath
Date: Mon, 02 Oct 1995 00:00
Date: Mon, 02 Oct 1995 00:00
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Hi, I was wondering if anyone here uses precast gels for PAGE or even for agarose gels. I'm trying to get information about what sort of products are out there and what people like and don't like. If you do, please email me (or call me at 312-551-3833). I'd be very grateful for any feedback that you might have. I'm in a real hurry, so any information would be appreciated. Thanks, Michael -- ++ ++ --+- +-- Michael J. Fath | \/ | | |_ Department of Molecular Genetics and Cell Biology | | \_/ | The University of Chicago Chicago, IL 60637 mjfath@midway.uchicago.edu
Re: Mol Biologists, do any of you use precast gels?
Author: Paul D. Boyer
Date: Mon, 02 Oct 1995 00:00
Date: Mon, 02 Oct 1995 00:00
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I have a lot of experience with Novex' PAGE gels. I love them, although they're a bit pricey. I've never had a problem with them, and all you do is rip open the bag, pull the comb, and run. I can't rave enough about them. I only wish budgets these days allowed for them on a regular basis! -- ************************************************************************* Paul D. Boyer, Ph.D. 900 Wood Road, Box 2000 Assistant Professor Kenosha, WI 53141-2000 Department of Biological Sciences pboyer@cs.uwp.edu University of Wisconsin-Parkside *************************************************************************
Re: Mol Biologists, do any of you use precast gels?
Author: Anton Hutticher
Date: Thu, 05 Oct 1995 00:00
Date: Thu, 05 Oct 1995 00:00
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mjfath@ellis.uchicago.edu (Michael J. Fath) wrote: > > Hi, > > I was wondering if anyone here uses precast gels for PAGE > or even for agarose gels. (snip) We compared NOVEX precast PAGE gels with our gels. The resolution was quite good but not as good as our own specially crafted gradient gels. However, they were very easy to setup and use. Several people switched to NOVEX, other stayed with their own gels. If you do PAGE only sporadically, precasts are probably a good solution. Take care if you want to do western blots with precasts. Those from NOVEX we used could not stand the blotting conditions we used ( 1 l MeOH, 4,1 g NaHCO3, 1,6 g Na2CO3 ), 40 V, 3 h on Biorad Mini. They fragmented during the run and the fragments were very hard to peel off the nitrocellulose. We have not tested them under any other conditions! Maybe you find blotting conditions which work for them, but test them beforehand! Anton Hutticher (huttich@edvz.sbg.ac.at) (Uni Salzburg / Austria)
Re: Mol Biologists, do any of you use precast gels?
Author: Tomas Drgon
Date: Thu, 05 Oct 1995 00:00
Date: Thu, 05 Oct 1995 00:00
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In article <4516pc$k6@dwst13.edvz.sbg.ac.at>, Anton Hutticher <huttich@edvz.sbg.ac.at> wrote: > > We compared NOVEX precast PAGE gels with our gels. The > resolution was quite good but not as good as our own specially > crafted gradient gels. However, they were very easy to setup > and use. Several people switched to NOVEX, other stayed with > their own gels. If you do PAGE only sporadically, precasts are > probably a good solution. > Take care if you want to do western blots with precasts. Those > from NOVEX we used could not stand the blotting conditions we > used ( 1 l MeOH, 4,1 g NaHCO3, 1,6 g Na2CO3 ), 40 V, 3 h on > Biorad Mini. They fragmented during the run and the fragments > were very hard to peel off the nitrocellulose. We have not tested > them under any other conditions! Maybe you find blotting conditions > which work for them, but test them beforehand! > > Anton Hutticher > (huttich@edvz.sbg.ac.at) > (Uni Salzburg / Austria) I use Novex precast gels for westerns. They run great in Tris/Glycine/SDS and also in CAPS blotting buffers (200 mA for 2 hrs). I also occasionally use precast and pre-EtBr-stained agarose gels from FMC Bioproducts. They are also ok. Tomas Drgon -- Signature under construction...
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