Vital stains for bacteria

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2 total messages Started by Brian Robertson Wed, 08 Mar 1995 09:34

#26

Author: Brian Robertson
Date: Wed, 08 Mar 1995 09:34

25 lines
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I am looking for a fluorescent vital stain to assess bacterial viability
in the FACS.

Fluorescene Diacetate (FDA), which fluoresces when the fluorescein is
released by the action of nonspecfic bacterial esterases in live cells, is
one possiblity, but is the wrong colour.

Does anyone know if, for example, rhodamine or texas red diacetate, or
some other colour is available? Or if there is another dye system around
which gives a colour that could be measured in something other than the
FITC channel?

Many thanks!

Brian Robertson

-- 
Dr. Brian D. Robertson
Dept. Medical Microbiology
St Mary's Hospital Medical School
Norfolk Place
London W2 1PG
U.K.

b.robertson@sm.ic.ac.uk

Re: Vital stains for bacteria

#25

Author: Margaret Romine
Date: Thu, 09 Mar 1995 17:03

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In article <b.robertson-0803950934340001@cb-11.sm.ic.ac.uk>,
b.robertson@ic.ac.uk (Brian Robertson) wrote:

> I am looking for a fluorescent vital stain to assess bacterial viability
> in the FACS.
> 
> Fluorescene Diacetate (FDA), which fluoresces when the fluorescein is
> released by the action of nonspecfic bacterial esterases in live cells, is
> one possiblity, but is the wrong colour.
> 
> Does anyone know if, for example, rhodamine or texas red diacetate, or
> some other colour is available? Or if there is another dye system around
> which gives a colour that could be measured in something other than the
> FITC channel?
> 
> Many thanks!
> 
> Brian Robertson
> 
> -- 
> Dr. Brian D. Robertson
> Dept. Medical Microbiology
> St Mary's Hospital Medical School
> Norfolk Place
> London W2 1PG
> U.K.
> 
> b.robertson@sm.ic.ac.uk

Try molecular Probes, Inc. at 503-465-8300.  They have a large variety of
fluorescent probes.

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