🚀 go-pugleaf

First of Three of Six wrote:
> Hi can anyone share tips and tricks on capturing images of bacteria stained 
> by Gram's method.
> I'm using an Olympus BX51 with a DP70 and capturing the images using Olysia 
> BioReport.
> While I'm finding the process excellent for larger organisms via a 40 
> objective, when using oil 100 focusing and sharpness have suffered.
> Everything looks good on the screen, but then I transfer the image (saved as 
> tif and usually around 36 MB) 

What are you doing with them? You probably dont need to photograph them 
at such high resolution (well, with such large file sizes I should say).

 >
> and attempt some manipulation with Photoshop 
> to resize to 96 dpi and save as jpg (10/10 quality) the picture degrades a 
> little.
 >

Why do you want them at 96dpi? Do you need large image sizes? You may 
find that the images are better when recorded in lower resolutions to 
begin with if you dont need the print size.

 >
> Thanks in advance. I'm mostly keen to get any help on the best focussing 
> techniques people use.  
> 

Well, for focussing, I just focus until it's as sharp as I can get. Not 
much you can do once you've done that.

Scott.

"Scott Coutts" <scott.coutts@med.monash.edu.au> wrote in message 
news:uQ6Ae.36633$oJ.18937@news-server.bigpond.net.au...
> First of Three of Six wrote:
>> Hi can anyone share tips and tricks on capturing images of bacteria 
>> stained by Gram's method.
>> I'm using an Olympus BX51 with a DP70 and capturing the images using 
>> Olysia BioReport.
>> While I'm finding the process excellent for larger organisms via a 40 
>> objective, when using oil 100 focusing and sharpness have suffered.
>> Everything looks good on the screen, but then I transfer the image (saved 
>> as tif and usually around 36 MB)
>
> What are you doing with them? You probably dont need to photograph them at 
> such high resolution (well, with such large file sizes I should say).
>
> >
>> and attempt some manipulation with Photoshop to resize to 96 dpi and save 
>> as jpg (10/10 quality) the picture degrades a little.
> >
>
> Why do you want them at 96dpi? Do you need large image sizes? You may find 
> that the images are better when recorded in lower resolutions to begin 
> with if you dont need the print size.
>
> >
>> Thanks in advance. I'm mostly keen to get any help on the best focussing 
>> techniques people use.
>
> Well, for focussing, I just focus until it's as sharp as I can get. Not 
> much you can do once you've done that.

Now that memory is so cheap I record the material at the best quality I can 
and use photoshop to resize for a variety of purposes. I usually resize to 
20 cm by 15 cm and 72 or 96 dpi for Intranet pages that I use for education.
For printing (examinations, quality assurance or just for fun) I resolve to 
300 and vary the size according to the print size I want. These smaller 
image files are usually deleted and I retain the original file on a server 
and portable media. I don't keep everything, just the images I like. This 
doesn't just apply to photomicroscopy but all forms of image capture.

Thanks for answering my questions, I just wanted to make sure I was doing 
everything possible to optimise my material.

As for capture as a lower magnification, it's a bit hard to see things like 
polar staining or vacuolation in a bacterium at less than using a 100 oil 
immersion objective.

First of Three of Six wrote:
> "Scott Coutts" <scott.coutts@med.monash.edu.au> wrote in message 
> news:uQ6Ae.36633$oJ.18937@news-server.bigpond.net.au...
> 
>>First of Three of Six wrote:
>>
>>>Hi can anyone share tips and tricks on capturing images of bacteria 
>>>stained by Gram's method.
>>>I'm using an Olympus BX51 with a DP70 and capturing the images using 
>>>Olysia BioReport.
>>>While I'm finding the process excellent for larger organisms via a 40 
>>>objective, when using oil 100 focusing and sharpness have suffered.
>>>Everything looks good on the screen, but then I transfer the image (saved 
>>>as tif and usually around 36 MB)
>>
>>What are you doing with them? You probably dont need to photograph them at 
>>such high resolution (well, with such large file sizes I should say).
>>
>>
>>>and attempt some manipulation with Photoshop to resize to 96 dpi and save 
>>>as jpg (10/10 quality) the picture degrades a little.
>>>
>>
>>Why do you want them at 96dpi? Do you need large image sizes? You may find 
>>that the images are better when recorded in lower resolutions to begin 
>>with if you dont need the print size.
>>
>>
>>>Thanks in advance. I'm mostly keen to get any help on the best focussing 
>>>techniques people use.
>>
>>Well, for focussing, I just focus until it's as sharp as I can get. Not 
>>much you can do once you've done that.
> 
> 
> Now that memory is so cheap I record the material at the best quality I can 
> and use photoshop to resize for a variety of purposes. I usually resize to 
> 20 cm by 15 cm and 72 or 96 dpi for Intranet pages that I use for education.
> For printing (examinations, quality assurance or just for fun) I resolve to 
> 300 and vary the size according to the print size I want. These smaller 
> image files are usually deleted and I retain the original file on a server 
> and portable media. I don't keep everything, just the images I like. This 
> doesn't just apply to photomicroscopy but all forms of image capture.
> 
> Thanks for answering my questions, I just wanted to make sure I was doing 
> everything possible to optimise my material.
> 
> As for capture as a lower magnification, it's a bit hard to see things like 
> polar staining or vacuolation in a bacterium at less than using a 100 oil 
> immersion objective.
> 

Yeah, the only reason I suggested lower resolution is because the camera 
is actually only a 1.5MP sensor, and it can sometimes produce better 
images at lower resolutions, rather htan higher res where it has to do 
that shifting thing that it does to get it's equivalent (not acutal) 
12MP. Usually it's aproblem with moving objects, though.

"First of Three of Six" <firstofthreeofsix@hotmail.com> wrote in message 
news:Dv6Ae.36608$oJ.11643@news-server.bigpond.net.au...
> Hi can anyone share tips and tricks on capturing images of bacteria 
> stained by Gram's method.
> I'm using an Olympus BX51 with a DP70 and capturing the images using 
> Olysia BioReport.
> While I'm finding the process excellent for larger organisms via a 40 
> objective, when using oil 100 focusing and sharpness have suffered.
> Everything looks good on the screen, but then I transfer the image (saved 
> as tif and usually around 36 MB) and attempt some manipulation with 
> Photoshop to resize to 96 dpi and save as jpg (10/10 quality) the picture 
> degrades a little.
> Thanks in advance. I'm mostly keen to get any help on the best focussing 
> techniques people use.

you might want to check a few things in photoshop if u having grungy 
resizing issues.  look in preferences, and make sure that IMAGE 
INTERPOLATION is set to no less then BICUBIC (SMOOTHER).  once that's set, 
now rezise it again and see.

another thing too is that you might want to view your files at 100% 
resolution, or u'll confuse yourself.

when you saving jpg also, u can do 300dpi, i'd suggest doing 300dpi.  works 
good both for prints and also if need be easy to convert to 72 web size. 

"First of Three of Six" <firstofthreeofsix@hotmail.com> wrote in message
news:Dv6Ae.36608$oJ.11643@news-server.bigpond.net.au...
> Hi can anyone share tips and tricks on capturing images of bacteria
> stained by Gram's method.
> I'm using an Olympus BX51 with a DP70 and capturing the images using
> Olysia BioReport.
> While I'm finding the process excellent for larger organisms via a 40
> objective, when using oil 100 focusing and sharpness have suffered.
> Everything looks good on the screen, but then I transfer the image (saved
> as tif and usually around 36 MB) and attempt some manipulation with
> Photoshop to resize to 96 dpi and save as jpg (10/10 quality) the picture
> degrades a little.
> Thanks in advance. I'm mostly keen to get any help on the best focussing
> techniques people use.

you might want to check a few things in photoshop if u having grungy
resizing issues.  look in preferences, and make sure that IMAGE
INTERPOLATION is set to no less then BICUBIC (SMOOTHER).  once that's set,
now rezise it again and see.

another thing too is that you might want to view your files at 100%
resolution, or u'll confuse yourself.

when you saving jpg also, u can do 300dpi, i'd suggest doing 300dpi.  works
good both for prints and also if need be easy to convert to 72 web size.